Fig. 2
In situ analysis of PTPRG expression. a Immunohistochemistry using monoclonal TPγ B9-2 and chPTPRG antibodies on cryostatic sections of mouse testis. In the heterozygous PTPRG (+/−) mice, shown in the insets, the two antibodies recognize the same structures while in Ptprg-null (KO) (−/−) mice, shown as the main figure, no staining was detectable. b Comparison between TPγ B9-2, chPTPRG, and RbtP4 in normal human lung (a–c) and pancreas (d–f). Formalin-fixed, paraffin-embedded sections were stained with anti-PTPRG antibodies indicated. All the antibodies recognize lung alveolar macrophages (a–c, arrows). In pancreas (d–f) TPγ B9-2 preferentially stains Islets of Langerhans (white arrows) and centroacinar regions (black arrows), while chPTPRG stains the tissue more diffusely, including the exocrine glands. Matched irrelevant antibodies controls (murine IgG1, IgY, and rabbit IgG) are shown in the insets