Fig. 4
Flow cytometric analysis of healthy human donor peripheral blood samples using two anti-PTPRG antibodies. Dot plots show gating strategy for each cell subset analyzed in histograms. N = 34 monocytes, N = 20 T cells, N = 26 B cells, N = 16 neutrophils, N = 10 eosinophils, and N = 18 CD34+ cells. PTPRG expression in peripheral blood circulating monocytes (CD45+; CD14+), T cells (CD45+; CD3+), B cells (CD45+; CD19+), PMN neutrophils (CD45+; CD16+), PMN eosinophils (CD45+; CD16−), CD34+ progenitor cells (CD45low; CD34+), using the reference antibody chPTPRG and TPγ B9-2. Data are expressed as mean fluorescence intensity (MFI) with appropriate isotype controls (preimmune chicken IgY and irrelevant murine IgG1) as references