Fig. 4

K331 and K345 are critical for UBE2O-mediated c-Maf degradation. a Wild-type and lysine residual mutated c-Maf plasmids were individually transfected into HEK293T cells with or without UBE2O. Twenty-four hours later, cell lysates were prepared for immunoblotting against specific antibodies as indicated. b The plasmids of K331R or K345R c-Maf mutants were co-transfected with increased UBE2O for 24 h. Wild-type (WT) and all lysine residual mutant (KO) c-Maf were used as positive and negative control. c The plasmids of K331R and K345R c-Maf mutants or control plasmids were co-transfected into HEK293T cells, respectively, with a Ub plasmid for 24 h, followed by IP and immunoblotting assays as indicated