Fig. 4

Networks predicted by Ingenuity Pathway Analysis in the LIN28B knocking down cells. a Top five canonical pathways affected by silencing LIN28B. b The interaction network displayed graphically as nodes (genes) was centered on JUN and LIN28B. The coding color and lines are defined by IPA software by default. The node color intensity indicates the expression of genes, with red representing upregulation and green representing downregulation. Solid lines and dotted lines indicate direct relationship and indirect relationships, respectively. c The measurements of glutamine, l-amino acid, and aspartate in TF-1a-Scramble shRNA, TF-1a-LIN28B-shRNA3, and TF-1a-LIN28B-shRNA5 cell lysates. The relative fold changes were compared to the amount of these three metabolites in TF-1a-Scramble shRNA cell lysate, which were set as 1. The experiments were triplicated (mean ± SD). *p < 0.05; **p < 0.01