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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: The distinct biological implications of Asxl1 mutation and its roles in leukemogenesis revealed by a knock-in mouse model

Fig. 7

Gene expression and gene set analyses of the interplay between ASXL1 mutations and MN1 overexpression. a Proportions of significantly differentially expressed genes and b enriched gene sets between samples with mutant and wild-type ASXL1 in mice and AML patients. Significant differential expression was defined by Student’s t test p < 0.05; gene set enrichment analysis was conducted by GSEA, with a threshold on GSEA p value at 0.0005. cf GSEA enrichment plots of selected oncogenic gene signatures. For each microarray dataset, all genes were sorted by the significance of differential expression between samples carrying mutant and wild-type ASXL1 (denoted by left and right arrows). Gene sets were tested for overrepresentation at either side of the ranked list, of which the overrepresentation was measured by a running enrichment score (ES; green curves). Positive and negative ES represent enrichments in ASXL1-mutated and wild-type samples, respectively. Significance of an ES was assessed by random permutation of the gene list. c, d All of the five gene signatures showed MN1-transduction specific enrichments in mice. e, f Concordant enrichments were seen in AML patients, with no dependency on the expression of MN1. These oncogenic functions may partially account for the change in the threshold of MN1-driven engraftment in the presence of Asxl1 mutation

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