Fig. 8

ChIP-Seq analysis of H3K27me3 in Asxl1 ^tm/+ and wild-type bone marrow cells. a Integrative Genome Viewer plots of Asxl1 ^tm/+ and wild-type specific peaks in the mouse genome. Bottom panels, enlarged plots of two examples. b Distributions of the Asxl1 ^tm/+ modulated peaks in gene regions. Upstream and downstream lengths were set at 5 k and 1 k bps., respectively. The specific H3K27 trimethylation peaks are significantly enriched in upstream and downstream regions. *, binomial test p < 0.05; ***, p < 0.001. c Top enriched motifs on the Asxl1 ^tm/+ modulated peaks reported by the MEME-ChIP web tool. d Proportions of significant downregulated genes in Asxl1 wild-type (upper panel) and Asxl1 ^tm/+ (lower) cells harboring condition-specific H3K27me3 peaks. 15.69% of the Asxl1 wild-type-specific downregulated genes harbored wild-type-specific H3K27 trimethylation peaks, while only 11.69% of other genes in Asxl1 wild-type cells harbored wild-type-specific H3K27 trimethylation peaks. **, Fisher’s exact test p < 0.01; N.S., non-significant p