Fig. 3

TFAP2A promote transcription of TGFB1 in CCA. a The 3′-UTR of TFAP2A exists a binding site of miR-200b; HCCC cell-line was transfected with full-length 3′-UTR (wild-type or mutant) of TFAP2A, and the luciferase reporter was performed to confirm the direct target sites. b The mRNA expression of TFAP2A was determined in CCA tissues by Q-PCR. c The luciferase reporter was performed to confirm that AP-2α as a transcription factor could promote the transcription of TGFB1. d HCCC cells were treated with or without TGF-β and then EMSA experiments were performed to test the ability of AP-2α to directly bind to the TGFB1 promoter. e-f HCCC cells were overexpressed with miR-200b and TFAP2A, and the MET phenotype were determined by Q-PCR. *p < 0.05, **p < 0.01, ***p < 0.001, data represent the mean ± SD