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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia

Fig. 3

Engraftment of AML cells is more efficient in newborn NSG pups than adult NSG mice. Magnetically sorted CD34+ pooled BM cells and splenocytes from secondary engrafted NSG mice were injected intrahepatically in newborn NSG pups (1Gy; n = 5) or intravenously in 6-week-old NSG adults (2.5Gy; n = 5) after sublethal irradiation (1 × 105 cells per mice). a Survival curve of newborn NSG pups and adult NSG mice over 20 weeks. b Comparison of longitudinal AML engraftment in peripheral blood between newborn NSG pups and adult NSG mice. Data are presented as mean absolute human CD45+ cell count per microliter of blood ± SEM. Two-tailed Mann Whitney U test; *p < 0.05. c Comparison of pathological outcome and spleen size between engrafted newborn NSG pups and adult NSG mice at endpoint (week 17–20 post-engraftment). Representative images of abdomen and spleen were shown; scale bar: 1 cm. The weight of spleen is presented as mean ± SEM. Two-tailed Mann Whitney U test; *p < 0.05. d Comparison of absolute count of human CD45+ cells (d, above) and CD34+ cells (d, below) in peripheral blood, spleen, and BM between newborn NSG pups and adult NSG mice at endpoint (week 17–18 post-engraftment). Data are presented as mean absolute count ± SEM. Two-tailed Mann Whitney U test; *p < 0.05

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