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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia

Fig. 4

CD117 expression enhances AML engraftment but is not essential to initiate leukemia. Pooled BM cells and splenocytes from primary engrafted NSG mice were magnetically sorted to CD34+CD117+ and CD34+CD117 AML cells. a Methocult™ colony-forming unit (CFU) assay. Representative images at 3 weeks post-culture were shown. Colonies were counted microscopically and the mean number of colonies obtained in duplicate dishes (1 × 104 cells inoculated per dish) was indicated. b, c Vary number of either CD34+CD117 or CD34+CD117+ AML cells (1 × 104, 5 × 104 and 1 × 105) were injected intrahepatically after sublethal irradiation in newborn NSG pups (n = 5 per group). Representative flow cytometry plots (b), frequency (c, above), and absolute count (c, below) of AML engraftment in peripheral blood at week 12 post-engraftment. Data are presented as mean frequency or absolute count of human CD45+ cells ± SEM. Two-tailed Mann Whitney U test; *p < 0.05, **p < 0.01. d Comparison of AML engraftment at endpoint (week 16 post-engraftment) in peripheral blood, spleen, and BM between newborn NSG pups engrafted with 1 × 105 of CD34+CD117+ and CD34+CD117 AML cells. Data are presented as mean frequency (d, above) or absolute count (d, below) of human CD45+ cells ± SEM. Two-tailed Mann Whitney U test; *p < 0.05. e, f Immunophenotypic analysis of CD34+CD117+ and CD34+CD117 engrafted recipient mice. Frequency e and absolute count f of CD34 CD117 , CD34 CD117 +, CD34 + CD117 +, and CD34 + CD117 subsets in peripheral blood, spleen, and BM at week 16 post-engraftment. Data are presented as mean frequency of human CD45+ cells or absolute count ± SEM. Two-tailed Mann Whitney U test; *p < 0.05, **p < 0.01

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