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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Kinase profiling of liposarcomas using RNAi and drug screening assays identified druggable targets

Fig. 5

Ponatinib causes cell cycle arrest and apoptosis of liposarcoma cells. a Flow cytometric cell cycle analysis of ponatinib (1000 nM, 16 h)-treated LPS141 and MLS402 liposarcoma cells compared to diluent control. Bar graphs show the percentage of cells in different phases of the cell cycle. Experiments were done in triplicates, repeated 3 times and results represent mean ± SE. b Western blot analysis of cell cycle-related proteins in LPS141 and MLS402 cells treated with ponatinib (250–1000 nM, 16 h) or diluent control (β-actin, loading control). c Flow cytometric Annexin V-APC/Propidium iodide staining of LPS141 and MLS402 cells treated with ponatinib (1000 nM, 16 h). Percentage of Annexin V+/PI+ cells (apoptotic cells) of ponatinib- and diluent control-treated cells shown in bar graphs. Experiments were done in triplicates, repeated 3 times and results represent mean ± SE. *P value ≤ 0.01. d Proteins associated with growth and apoptosis were analyzed by western blotting in liposarcoma cells treated with either ponatinib or diluent control (GAPDH, loading control)

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