Fig. 6
From: Kinase profiling of liposarcomas using RNAi and drug screening assays identified druggable targets
Ponatinib inhibits growth of liposarcoma xenografts. a LPS141 cells (2 × 106) mixed with equal volume of matrigel were injected subcutaneously in the flank of NSG mice. When tumors reached ~ 100 mm3, mice were randomly divided into two groups [experimental (n = 9) and control (n = 0)]. Experimental group received daily ponatinib (10 mg/kg), and control group received an equal volume of the vehicle, both by oral gavage for 21 days. Images of dissected tumors from mice of both groups are shown. b Bar graphs of tumor weights from vehicle- and ponatinib-treated mice. Results represent mean ± standard deviation. *P value ≤ 0.01. c Immunohistochemical staining for Ki-67 (proliferation marker) on tumor sections of mice treated with either vehicle or ponatinib. d Western blot analysis of phosphorylated and total protein levels of KIT and FGFR in the xenograft tumors of vehicle- and ponatinib-treated mice (α-tubulin, loading control). e Apoptotic- and growth-associated proteins examined by western blotting from tumor lysates of the xenografts treated with either ponatinib or vehicle (α-tubulin, loading control)