Fig. 1

Ezh2 is upregulated in human GC tissues and associated with poor prognosis of GC. a The qRT-PCR results showed that the levels of Ezh2 mRNA in 156 pairs of GC tissues were significantly higher than those in adjacent normal mucosa. Data are represented as mean ± SD. b Representative band of Western blotting showed that Ezh2 protein expression in GC tissues was higher than those in adjacent normal mucosa. c Representative image and the percentage of gastric cancer or normal gastric tissues with low or high Ezh2 protein expression. d Correlation analysis showed a positive correlation between EZH2’s mRNA and protein expression in the same 20 cases. Data were analyzed by Spearman’s correlation. e Kaplan-Meier survival curves with a log-rank test in the IHC group showed that the OS (n = 105) rates of the high EZH2 expression group were significantly lower than that of the low expression group (P = 0.033). The HRs and P values were calculated with log-rank tests. f Kaplan-Meier survival curves showed poor disease-free survival (DFS) and overall survival in patients (FUSCC cohort, n = 156) with high expression of Ezh2 compared with those in the low expression group. HRs and P values were calculated with log-rank tests. g Kaplan-Meier survival curves showed poor disease-free survival (DFS, n = 359) and overall survival (n = 593) in patients (GEO cohort) with high expression of Ezh2 compared with those in the low expression group. HRs and P values were calculated with log-rank tests