Fig. 3

Ezh2 regulates the expression of EMT, pluripotent protein, and PTEN/AKT signaling in GC. a Representative images and the number of invasive cells per high-power field (100×) showed that cell invasiveness was promoted by overexpression of Ezh2 in MKN-45 and SGC-7901 cells but suppressed by Ezh2 knockdown in AGS cells. Data are represented as mean ± SEM. *P < 0.01. b Representative images and the number of sphere formation per high-power field (100×) showed that stem cell-like properties were promoted by overexpression of Ezh2 in MKN-45 and SGC-7901 cells but suppressed by Ezh2 knockdown in AGS cells. Data are represented as mean ± SEM. *P < 0.01. c Heat-map display showed that the Ezh2-altered genes involved in EMT and cell stemness a. Gene expression profiling was performed with mRNA sequencing. d Representative images of the Western blot analysis for expression of Ezh2, E-cadherin, Vimentin, Oct4, and Sox2 in Ezh2-overexpressing MKN-45 cells and normal control, as well as Ezh2-knockdown AGS cells and normal control. e Representative images of the IHC analysis for expression of Ezh2, E-cadherin, Vimentin, Oct4, and Sox2 in xenograft tissues (up) and expression of Ezh2, Oct4, Sox2, Bmi-1, and CD44 in human GC and normal gastric mucous (down)