Fig. 5

Ezh2 facilitated GC progression in part via PTEN/Akt pathway. a The CCK-8 results showed that overexpression of PTEN partially attenuated the enhanced cell proliferation induced by overexpression of Ezh2 in MKN-45 cells, while knockdown of PTEN partially rescued the decreased cell proliferation induced by knockdown of Ezh2 in MKN-45 cells. Data are represented as mean ± SEM. * P < 0.01. b Representative images (left) and the number of invading cells (right) per high-power field showed that upregulation of PTEN partially attenuated the enhanced invasiveness ability of MKN-45 cells promoted by Ezh2 overexpression, while downregulation of PTEN partially rescued the decreased cell invasiveness of AGC cells promoted by Ezh2 knockdown. Data are represented as mean ± SEM. * P < 0.01. c Representative images (left) and the number of sphere formation (right) per high-power field showed that upregulation of PTEN partially attenuated the enhanced sphere formation of MKN-45 cells promoted by Ezh2 overexpression, while downregulation of PTEN partially rescued the decreased sphere formation activity of AGS cells promoted by Ezh2 knockdown. Data are represented as mean ± SEM. * P < 0.01. d Ezh2-overexpressing MKN-45 cells were transfected with empty vector or LV-PTEN, while Ezh2-knockdown AGS cells were transfected with scrambled or PTEN siRNA, and cells were subjected to western blotting with the indicated antibodies