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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: CAR-T cells targeting CLL-1 as an approach to treat acute myeloid leukemia

Fig. 2

CLL-1 CAR construction and expression in primary human T cells. a Cell surface-binding of anti-CLL-1 antibody and CLL-1 scFv-Fc protein to U937 cells, anti-IgG Fc was used as the secondary antibody. b Schematic of the CLL-1 CAR vector containing the anti-human CLL-1 scFv linked to CD28, 4-1BB costimulatory domains, and CD3-ζ signaling domain. c Representative phenotype of non-transduced T cells (NT) and CAR-transduced T cells derived from a single healthy donor. CAR-modified T cells were stained with anti-CD3 and anti-Thy1.1 after immunomagnetic selection and 1 cycle of expansion. Anti-CD4 and anti-CD8 were analyzed as well. Percentages in each quadrant are indicated. d Expression of indicated cell surface markers from three different healthy donor T cell lines following immunomagnetic selection and 1 cycle of expansion. Data represent mean values ± standard deviation (SD). VH variable heavy chain, L linker, VL variable light chain, scFv single-chain variable fragment

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