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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: CAR-T cells targeting CLL-1 as an approach to treat acute myeloid leukemia

Fig. 5

CLL-1 CAR-T cells do not target hematopoietic stem cells. a Expression of CLL-1 in mature myeloid cells after co-cultured with CLL-1 CAR-T cells. CD34-enriched normal hematopoietic cells and granulocytes were stained with CLL-1 after 24-h co-cultured with CAR-T cells or NT cells. b Summary of the in vitro killing assay for normal mature myeloid cells and HSCs. Normal mature myeloid cells (CD33+SSChigh) and myeloid progenitor cells (CD34+CD38+/CD34+CD33+) were lysed by CLL-1 CAR-T cells. Normal HSCs (CD34+CD38−) were not targeted by CLL-1 CAR-T cells. Data represent mean values of triplicate wells ± SD. c CD34+ CB cells (n = 2) were CD34-immunomagnetically selected and co-cultured with either non-transduced- or CLL-1-specific pair-matched T cells from healthy donors or media alone for 4 h at an E:T of 10:1. The cells were then plated in semisolid methylcellulose-based growth medium for 14 days and counted for the presence of BFU-E, CFU-GM, and CFU-GEMM colonies. Data represent mean values ± SD for two different CB samples. *p < 0.05, NS not significant

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