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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: SF3B1 deficiency impairs human erythropoiesis via activation of p53 pathway: implications for understanding of ineffective erythropoiesis in MDS

Fig. 2

Effects of SF3B1 knockdown on terminal erythroid differentiation. a Flow cytometry analysis showing the percentage of GPA-positive cells on day 7. b Flow cytometry analysis showing the expression of α4 integrin and band 3 of erythroid cells cultured for different days as indicated. c Growth curves of cells transduced with lentivirus containing luciferase-shRNA or SF3B1-shRNA. d Representative flow cytometry profiles of apoptosis as assessed by dual staining of Annexin V and 7AAD of cells cultured for 9 days. e Quantitative analysis of apoptosis from three independent experiments. f Representative flow cytometry profiles of cell cycle as assessed by EdU and 7AAD staining of cells cultured for 7 days. g Quantitative analysis of cell cycle from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Hematology & Oncology

ISSN: 1756-8722

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