Fig. 5

Effects of SF3B1 knockdown on splicing in CFU-E cells. a The most frequent splicing events by type after SF3B1 knockdown: ESI, exon skipping/inclusion; MESI, multiple exon skipping/inclusion; ISI, intron skipping/inclusion; A5, alternative 5′ splice site; A3, alternative 3′ splice site; ATSS, alternative transcription start site; ATTS, alternative transcription termination site; MEE, mutually exclusive exons. b Expression of differentially spliced transcripts checked by real-time PCR. c Schematic expression of two MKRN1 transcripts. d Bar graphs showing the large and small isoforms of MKRN1 in luciferase control groups and SF3B1 knockdown groups by the analysis of RNA-seq data. Dot plots represent 3 independent experiments