Fig. 7

Downregulation of mitosis/cytokinesis pathway in polychromatic and orthochromatic erythroblasts. a RNA-seq expression data of RACGAP1, PRC1, and PLK1 in luciferase and SF3B1 knockdown polychromatic and orthochromatic erythroblasts. Dot plots represent 3 independent experiments. b mRNA expression levels of RACGAP1, PRC1, and PLK1 as assessed by real-time PCR using β-actin as internal calibrator. c Representative images of morphology from DMSO and PLK1 inhibitor treated cells. d Quantification of abnormally nucleated erythroblasts from three independent experiments. **P < 0.01