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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: CBX7 regulates stem cell-like properties of gastric cancer cells via p16 and AKT-NF-κB-miR-21 pathways

Fig. 1

CBX7 positively regulates stem cell-like properties of gastric cancer cells. a Tumorigenic spheres (aka GCSC spheres) derived from SGC7901 gastric cancer cell line were cultured in serum-free DMEM/F12 medium supplemented with N2, B27, EGF, and bFGF. b Tumorigenic spheres overexpress stem cell markers including CBX7, OCT-4, CD44, and CD24. The expression of stem cell markers in the total cell lysate was analyzed by Western blot analysis. c The levels of CBX7 and stem cell markers OCT-4, CD44, and CD24 were detected in SGC-7901 cells expressing control vector (NC) or exogenous CBX7 (CBX7-oe) using Western blot analysis. β-actin was used as an internal control. d Cell proliferation of control and CBX7-overexpressing cell lines was determined using CCK-8 assay. e Colony-forming capability of CBX7 overexpressing and control cells was determined by seeding 1000 cells in sphere-initiating medium and an incubation period of 10 days. The colonies were fixed and stained with crystal violet. f Compared with control cells, CBX7-overexpressing SGC-7901 cells showed enhanced resistance to Adriamycin (ADM) and 5-fluorouracil (5-Fu). Cells were treated with ADM and 5-Fu at the indicated concentrations, and the numbers of viable cells were counted after 72 h of the treatment. The percentage of viable cells is shown relative to the untreated controls. g The migration and invasion of CBX7-overexpressing and control cells were determined using Corning Chambers. h Representative images of GCSC spheres originated from control and CBX7-overexpressing SGC-7901 cells. i CBX7 overexpression enhances in vivo tumorigenicity of SGC7901 cells. In vivo tumorigenic capacity of control (Ctrl) and CBX7-overexpressing SGC7901 cells was examined using SCID mice xenograft tumor model. Error bars in all panels represent the mean ± SD. *P < 0.05, compared with the control group

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