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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: CBX7 regulates stem cell-like properties of gastric cancer cells via p16 and AKT-NF-κB-miR-21 pathways

Fig. 2

CBX7 regulates stem cell-like characteristics of gastric cancer cells through the inhibition of p16 (aka p16INK4a). a Stable overexpression of CBX7 downregulates the expression of p16 in GC cells. Western blot analysis was used to determine the levels of CBX7, p16, and β-actin in control and CBX7-overexpressing SGC-7901 and AGS cells. β-actin was used as an internal control. b Depletion of CBX7 upregulates p16. Western blot analysis was employed to detect CBX7 and p16 expression in SGC-7901 cells after transfection with p16 shRNA, CBX7 shRNA, or control construct. β-actin was used as an internal control. cf Knockdown of p16 overcomes CBX7-induced GCSC phenotypes, as determined by colony formation (c), spheroid formation (d), migration and invasion (e), and chemoresistance assays (f). SGC-7901 cells were used to examine GCSC properties as described. EPI epirubicin. Error bars in all panels represent the mean ± SD (*P < 0.05, **P < 0.01 and ***P < 0.001, compared with the control)

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