Fig. 3

Efficacy of volitinib on PDX models with the corresponding expression of MET and pMET. a Volitinib showed significant antitumor activity in three out of seven PDX models (n = 5 per group). Tumor volumes and proportion of tumor growth inhibition were expressed as means ± SD. NS, p > 0.05; ***p < 0.001 according to repeated measures ANOVA. b, c The MET and pMET expression of corresponding PDX models assessed by IHC and immunoblot. Scale bar represents 100 μm. d The immunoblot analysis of critical molecules in the PI3K/AKT pathway before and after treatment with volitinib. e Quantification and normalization of immunoblot bands of pMET, pAKT, and pERK. *p < 0.05, ***p < 0.001 according to unpaired two-tailed t test. f Immunohistochemical analysis showed that volitinib reduced the level of phosphorylated MET, AKT, ERK, and S6 in case 156. Scale bar represents 100 μm