Fig. 3

NLRX1 suppresses EMT via the AKT-Snail1 axis. a Typical images of HCC cells receiving NLRX1 modulation. b Expression of E-cadherin, N-cadherin, Vimentin, Snail1, and Twist1 after NLRX1 modulation in Huh7 and HCCLM3 cells detected by RT-PCR. c Expression of E-cadherin, N-cadherin, Vimentin, Snail1, and Twist1 after NLRX1 modulation in Huh7 and HCCLM3 cells detected by WB. d Results of Cignal Finder RTK signaling 10-Pathway Reporter array conducted in Huh7 and HCCLM3 cell lines. e Phosphorylation of AKT and mTOR after NLRX1 OE in Huh7 cells detected by WB. f Evaluation of Snail1 expression level and phosphorylation level of AKT after treating NLRX1-OE HCCLM3 cells with an AKT activator, SC79, by WB assays. g Evaluation of E-cadherin and N-cadherin expression levels after treating NLRX1-OE HCCLM3 cells with an AKT activator, SC79, by RT-PCR. Asterisk indicates P < 0.05