Fig. 3

Loss of CAMKIV decreases self-renewal capacity of AML-SCs. a, b Representative flow cytometry plots show that Camk4-null MLL-AF9 AML mice had decreased numbers of YFP⁺Mac-1⁺Kit⁺ progenitors in both primary and secondary transplants. c, d Primary and secondary transplants with Camk4-null MLL-AF9 BM cells show significantly decreased colony-forming ability (n = 3). e Camk4-null MLL-AF9 BM cells show increased apoptosis. AnnexinV⁺/7AAD⁻ indicates early apoptosis, and AnnexinV⁺/7AAD⁺ indicates late apoptosis. *p < 0.05. f Limiting dilution assays comparing the frequencies of AML stem cells in WT and Camk4-null MLL-AF9 AML. The indicated YFP⁺ WT and Camk4-null MLL-AF9 BM cells that were collected from primary recipients were co-transplanted with 2 × 10⁵ bone marrow competitor cells into lethally irradiated recipients. The CRUs were calculated by L-Calc software