Fig. 5

Genetic deletion of thioredoxin using shRNA triggers mitophagy in BTZ-resistant multiple myeloma cells. a Western blot analyses of LC3B and PINK1 expression in MM.1R- and RPMI8226/Dox-BTZ-resistant cells stably transfected with shNT and shTNX. b JC-1 fluorescence intensity in MM.1R-BTZ-resistant cells stably transfected with shNT and shTNX, and treated with or without BTZ (5 nM). c JC-1 fluorescence intensity in RPMI8226/Dox-BTZ-resistant cells stably transfected with shNT and shTNX and treated with or without BTZ (5 nM). The intensity of expression was semi-quantitated using Image-Pro Plus 6.0 software and adjusted to β-actin. Error bars, standard error of the mean (SEM); *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001