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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: The proto-oncogene Mer tyrosine kinase is a novel therapeutic target in mantle cell lymphoma

Fig. 2

MerTK knockdown mediated by shRNA suppressed downstream signaling pathways, proliferation, and invasion in MCL cells. a MerTK knockdown by shRNA was validated by western blot in Z-138, Mino, and JVM-2 cells. Actin is shown as a loading control. b Downstream signaling in MerTK knockdown cell lines was evaluated by western blot. Western blot indicated that phosphorylation of AKT (p-AKT) and p38 (p-p38) was inhibited in Z-138, Mino, and JVM-2 cells infected with shMerTK compared to that in cells infected with shControl. c Proliferation of Z-138, Mino, and JVM-2 cells infected with shMerTK was significantly suppressed compared to that in the shControl group. d MerTK knockdown significantly suppressed invasive abilities of Z-138, Mino, and JVM-2 cells. Invasive abilities were determined by the number of viable cells invading into the lower chamber of the transwell chambers coated with Matrigel inserted in 24-well plates. Images from a representative experiment are shown. Mean values and standard errors (SEs) were derived from at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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