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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: The proto-oncogene Mer tyrosine kinase is a novel therapeutic target in mantle cell lymphoma

Fig. 4

UNC2250 prompted apoptosis and induced G2/M phase arrest in MCL cells. a UNC2250 prompted apoptosis in Z-138, Mino, and JVM-2 cells. Cells pre-treated with indicated concentrations of UNC2250 for 24 and 48 h were stained with annexin-V-FITC and propidium iodide (PI). Then, apoptosis assays were performed by flow cytometry. Apoptosis cells were determined by FITC+ PI− cells and FITC+ PI+ cells. b UNC2250 mediated alterations in proteins associated with apoptosis in Z-138, Mino, and JVM-2 cells. Cells were treated with indicated concentrations of UNC2250 for 24 h; then, proteins associated with apoptosis were detected by western blot. c UNC2250 induced G2/M phase arrest in MCL cells. Z-138, Mino, and JVM-2 cells were treated with indicated concentrations of UNC2250 for 24 h. Then, cell cycle analysis was performed by flow cytometry. d UNC2250 mediated alterations in proteins associated with cell cycle in Z-138, Mino, and JVM-2 cells. Cells were treated with indicated concentrations of UNC2250 for 24 h; then, proteins associated with cell cycle were detected by western blot. Mean values and SEs were derived from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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