Fig. 1

B3GALNT2 is upregulated in hepatocellular carcinoma and correlated with poor prognosis. a Relative mRNA expression of B3GALNT2 was determined by real-time PCR in 24 pairs of HCC tumor tissues and adjacent non-tumor tissues. b Western blot analysis was used to determine the protein expression level of B3GALNT2 in 24 pairs of HCC tissues. Six representative pairs are shown. T HCC tumor tissue, N adjacent non-tumor tissue. c Statistical data of Western blot analysis on HCC tumor tissues and adjacent non-tumor tissues. d Representative IHC staining of adjacent non-tumor tissue and tumor tissue with high or low levels of B3GALNT2 in HCC. Regional magnification images are shown below. e Statistical data of the IHC staining score. f Relative mRNA expression of B3GALNT2 in HCC tumor tissues and normal liver tissues obtained from GSE25097, GSE54236, and TCGA-LIHC datasets. g Correlation between B3GALNT2 expression and tumor size in IHC cohort. h, i Kaplan-Meier analysis for overall survival of HCC patients from IHC cohort and TCGA-LIHC dataset