Fig. 3

B3GALNT2 knockdown inhibits macrophage recruitment in HCC cells. a IHC analysis of B3GALNT2 and F4/80 levels in tumor tissues from orthotopic xenografts. Cell count of macrophages is shown below. b Representative IHC staining of B3GALNT2 and CD68 in human HCC tumor tissues. The correlation between B3GALNT2 and CD68 levels in the IHC cohort are shown below. c, d Macrophage recruitment assessment in transwell assays using HCC cell lines with B3GALNT2 overexpression or knockdown. Both representative transwell images (c) and statistical data (d) are shown. e, f Macrophage recruitment assessment in transwell assays using culture medium of HCC cells with B3GALNT2 overexpression or knockdown. Both the representative transwell images (e) and statistical data (f) are shown. *p < 0.05