Fig. 5

miR-153 overexpression within colon cancer cells enhances CAR T cells proliferation and attenuates tumor growth. a Cytokine production of CAR T cells co-cultured with DLD-1 cells with or without miR-153. Gram-B granzyme B. P ≤ 0.05 in all three. b CFSE-based proliferation of CAR T cells cultured with DLD-1 + NC or DLD-1 + miR-153. NC is a normal control for DLD-1-miR-153 (DLD-1-luc cells were infected with virus carrying the parental vector). c Body weight of NSG mice injected with tumor cells and T cells. d Xenograft tumor growth in NSG mice inoculated with DLD-1 + NC or DLD-1 + miR-153 cells and treated with CAR T or WT T cells. At 5 days after inoculation with tumor cells, CAR T cells or WT T cells (1 × 10⁷) were injected intravenously, and tumor growth was monitored with luciferase-based imaging every 5 days for 15 days. *P ≤ 0.05; **P ≤ 0.01