Fig. 5

Effect of FLT3L CAR-T on STAT5, AKT, and ERK1/2 phosphorylation of FLT3⁺ leukemia cells. a FLT3 mutation types were assessed by PCR, using primers located in 14 and 15 exons as described in the “Methods” section. The upper arrow indicated the FLT3/ITD band, while the lower arrow pointed to the normal FLT3 band. b The fluorescence intensities of pSTAT5, pAKT, and pERK1/2 in CD33⁺leukemia cells were measured by intracellular staining with corresponding antibody and analyzed by using flow cytometry. Black histograms indicated fluorescence intensity of leukemia cells in the CAR-T group, while gray histograms indicated that of the VEC-T group. c SFI levels of pSTAT5, pAKT, and pERK1/2 obtained through dividing median fluorescence of leukemia cells in CAR-T group by median fluorescence of leukemia cells in VEC-T group