Fig. 6

FLT3L CAR T cells did not inhibit CD34⁺ cell colony formation. a The purity of CD34⁺ cells isolated from cord blood using the CD34 MicroBead Kit and expression of FLT3 on CD34⁺ cells were measured by flow cytometry. b Morphology of colonies (inverted microscope, × 10). CD34⁺ cells of cord blood (500 per well, from three different cord blood samples) co-cultured with VEC-T or CAR-T for 24 h, then treated or untreated CD34⁺ cells incubated in methylcellulose medium for 14 days. c Colony numbers of CD34+ cells per well