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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: miR-519a enhances chemosensitivity and promotes autophagy in glioblastoma by targeting STAT3/Bcl2 signaling pathway

Fig. 1

miR-519a sensitized GBM cells to TMZ treatment. a Parental (black ellipses) or resistant (black squares) cells were exposed to increase the concentrations of TMZ in serum-free medium (acute growth inhibition assay) for 72 h. Cell viability was measured by MTT assays. b The expression of miR-519a was detected by qRT-PCR. c Cell viability of DMSO- or TMZ-treated GBM cells transfected with miR-519a or anti-miR-519a. d, e GBM cells transfected with miR-519a or anti-miR-519a were treated with 400 μM TMZ for 36 h. Cells were harvested and stained with PI and annexin V-FITC for apoptotic analysis (**p < 0.01 vs. TMZ group). f, g Colony formation assay showing the sensitizing effects of miR-519a on GBM cells after TMZ treatment (**p < 0.01 vs. TMZ group). h Western blot analysis of cleaved caspase-3 in GBM cells transfected with miR-519a or anti-miR-519a and then treated with TMZ for 24 h. Data represent the mean (± standard deviations) and are representative of three independent experiments, each performed in triplicate. *p < 0.05, **p < 0.01

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