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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: miR-519a enhances chemosensitivity and promotes autophagy in glioblastoma by targeting STAT3/Bcl2 signaling pathway

Fig. 3

miR-519a sensitized GBM cells to TMZ treatment partly regulated by autophagy. U87-MG/TMZ cells transfected with miR-519a were treated with or without 400 μM TMZ after incubation with 3-MA (an inhibitor of autophagy) for 2 h. U87-MG cells transfected with anti-miR-519a were treated with or without 200 nM rapamycin for 2 h. The cells were then analyzed for the following: a assessment of proliferation by MTT assay; b, e assessment of colony formation; c, d assessment of apoptosis by FACS analysis of PI-stained cells; and f assessment of caspase-3 activity. *p < 0.05, **p < 0.01

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