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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties

Fig. 1

HepG2 cell-secreted exosomes are actively incorporated by adipocytes in culture. a A representative electron microscopy image of purified HepG2 exosomes showed morphology. Scale bar, 200 nm. b The nanoparticle size distribution for HepG2 exosomes was obtained by NTA. The particle size is between 0 and 300, with a peak around 100 nm. c Western blot analysis of exosome marker CD63, TSG101, and cell-specific marker calnexin. Positive control was HepG2 cell lysate, and negative control was culture medium. d Characterization of adipocytes differentiated from MSCs by morphology, Oil Red O staining, BODIPY staining, and expression of specific adipogenic marker genes CEBPɑ, PPARɤ, and LPL. e Adipocytes were incubated with 70 ng/mL PKH67-labeled HepG2 exosomes for the indicated times, and uptake of exosomes was determined by fluorescence confocal microscopy

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