Fig. 5

SNHG7 is a direct target of miR-34a and regulates GALNT7 expression in CRC cells. a Sequence alignment of miR-34a with the binding sites in the wild-type and mutant-type regions of SNHG7 was shown. b miR-34a expression in CRC tissues were determined. c The negative relevance between SNHG7 and miR-34a was revealed by Pearson’s correlation curve. d The relative luciferase activity of 293T cells was tested after co-transfection with SNHG7 wide-type and miR-34a mimic. e RIP assay was performed, and the co-precipitated RNA was subjected to qRT-PCR. RNA levels were presented as fold enrichment in Ago2 relative to IgG immunoprecipitates. f, g Relative GALNT7 expression of CRC tissues and CRC cells were analyzed. h The positive relevance between SNHG7 and GALNT7 expression was revealed by Pearson’s correlation curve. i GALNT7 level in SW480 cells transfected with SNHG7 was shown. j GALNT7 level in SW620 cells transfected with siSNHG7 was shown. The error bars in graphs represented SD, and each experiment was repeated thrice. *P < 0.05, **P < 0.01, ***P < 0.001