Fig. 2
From: Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells
Construction and expression of NKG2D CARs. a Schematic illustration of the lentiviral construct for the NKG2D CARs. NKG2D CAR sequences were preceded in frame by a green fluorescent protein (GFP) sequence followed by the 2A ribosomal skipping sequence. NKG2D CAR contains the extracellular portion of the human NKG2D receptor, which linked to a CD8a hinge and transmembrane region, followed by a CD3z signaling moiety alone (GFP-NKG2D-z) or in tandem with the 4-1BB (GFP-NKG2D-BBz) or CD27 (GFP-NKG2D-27z) intracellular signaling motif. b NKG2D CAR and GFP coexpressed by CD3+ T cells 14Â days after transduction. UNT T cells only express endogenous NKG2D