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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Fig. 1

The effects of Ube2v1 on autophagy program in colorectal cancer. a, b Protein expressions of LC3-II, Beclin1, and P62 level were examined by western blots when Ube2v1was overexpressed (a) or knocked down (b) in DLD-1 and SW480 cells under normal medium culture condition. c Protein expression of LC3-II was examined by western blots when Ube2v1was overexpressed in DLD-1 and SW480 cells under starvation in Hank’s buffered saline solution (HBSS). d Enlarged multivesicular structures corresponding to autophagic vacuole in cells with stable Ube2v1 overexpression under normal condition or starvation condition were observed by transmission electron microscope (TEM). e Immunofluorescence staining of endogenous LC3 or P62 puncta was analyzed in SW480 cells after Ube2v1 expression was knocked down. f A mCherry-GFP-LC3B reporter was transfected into the cells to monitor the autophagy progression from the autophagosome characterized with green fluorescent protein (GFP) to autolysosome characterized with red fluorescent protein (RFP) after Ube2v1 knockdown or and Torin (concentration) treatment for 12 h

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