Skip to main content
Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Fig. 3

The role of Ubc13 in Ube2v1 mediates Sirt1 ubiquitination in CRC cells. a Immunoprecipitation assay for the exogenous interaction between Ube2v1 and Sirt1in SW480 cells transfected with Flag-Ube2v1 and Myc-Sirt1. b Immunoprecipitation assay for the exogenous and endogenous interaction between Ubc13 and Sirt1 in SW480 cells. c Ubiquitination assays of endogenousSirt1 in the lysates from SW480 cells cotransfected with GFP-Ube2v1, HA-Ub, two siRNAs targeting Ubc13, or vector control. The cells were treated with or without MG132 (20 μM) before harvest and then immunoprecipitated them with anti-Sirt1 antibody. d Ubiquitination assays of endogenous Sirt1 in the lysates from SW480 cells cotransfected with GFP-Ube2v1, HA-Ub, NSC697923 (a small-molecule inhibitor targeting Ubc13), or DMSO control. The cells were treated with or without MG132 (20 μM) before harvest and then immunoprecipitated them with anti-Sirt1 antibody

Back to article page

Journal of Hematology & Oncology

ISSN: 1756-8722

Contact us