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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Fig. 4

The effects of Ube2v1 on epithelial mesenchymal transition and autophagy program in colorectal cancer. a, b Expressions of E-cadherin, β-catenin, Vimentin, Fibronectin, N-cadherin, Twist1, and Snai1were detected after Ube2v1 was overexpressed (a) or knocked down (b) in DLD-1 and SW480 cells. c Tumor exacted from mouse xenograft model were used to detect expressions of E-cadherin and LC3-II in both DLD-1 and SW480 cells with Ube2v1 stable overexpression by western blotting. d Endogenous LC3 puncta and expressions of E-cadherin, β-catenin, and Vimentin were observed after Ube2v1 was knocked down in SW480cells by immunofluorescent analysis. e Expressions of Ube2v1, ATG5, ATG7,LC3-II, E-cadherin, β-catenin, Vimentin, Fibronectin, and N-cadherin were observed after Ube2v1 was knocked down in HCT116 and SW480 cells with or without knockdown of ATG5 or ATG7 using RNA interference

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