Skip to main content
Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia

Fig. 2

AZD-1775 overrides the G2/M checkpoint and induces mitosis in B/T-ALL cell lines. a Viability analyses in ALL cell lines incubated for 24 h with AZD-1775 (6 to 5000 nM). The percentage of viable cells is depicted as a percentage of untreated controls. b Apoptosis analyses in BV-173, NALM-6, MOLT-4, and CCRF-CEM cells after 24 h of incubation with AZD-1775 (for each cell line: IC50, IC25, and IC12.5). The percentage of apoptotic cells was detected after Annexin V/propidium iodide staining. c Cell cycle analysis of BV-173 and CCRF-CEM cell line incubated for 24 h with increasing concentration of AZD-1775. d Representative immunoblots showing the expression of key proteins of the WEE1 pathway after treatment with AZD-1775 (IC50 for each cell line) for 24 h of the indicated cells lines. β-actin was used for loading normalization. e Quantitative mRNA analysis of six representative genes of 24 G2/M checkpoint genes analyzed. The white columns represent the controls and the gray columns represent the samples treated with AZD-1775 (IC50) for 12 h of the indicated cell lines. Results are expressed as Log10 2exp[−(ΔΔCt)

Back to article page