Fig. 4

AZD-1775 enhances the toxicity of antineoplastic compounds on ALL cell lines and primary cells. a Cell viability analyses of NALM-6, NALM-19, and REH cell lines incubated with AZD-1775 (6 to 5000 nM, dilution rate 1:3) and clofarabine (2.5, 5, and 10 nM) for 72 h. Viable cells are depicted relative to the untreated controls. Data were used to determine the CI values. b Growth curve of REH, NALM-6, CCRF-CEM, MOLT-4, and RPMI-8402 treated for 4 days with AZD-1775 (185 nM) and clofarabine (REH, NALM-6, and RPMI-8402 10 nM; CCRF-CEM and MOLT-4 20 nM). The number of viable cells was evaluated in the different groups every 24 h. c Apoptosis analyses of NALM-6 and MOLT-4 cell lines after 24 h of incubation with AZD-1775 (185 nM) and clofarabine (MOLT-4 20 nM and NALM-6 10 nM). The percentage of apoptotic cells was detected after Annexin V/propidium iodide staining. d Cell viability analysis on primary leukemic cells isolated from eight adult ALL patients treated with AZD-1775 (5 uM) and clofarabine (500 nM) for 24 h. Viable cells are depicted as percentage of the untreated controls. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001