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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: The CCR4-NOT complex is a tumor suppressor in Drosophila melanogaster eye cancer models

Fig. 5

Not3 reduced expression in the sensitized background drives oncogenic altered gene expression. a Workflow for the analysis of the RNA-sequencing experiments performed on fly eye-antennal eye discs. b Correlation matrix between the different samples, calculated on the normalized mRNA expression levels. c Volcano plot of all differential genes between sensitized + UAS-RNAi Not3 105990v and sensitized parental, which represents the results of three biological replicates. The other Not3 knockdowns show similar results. The genes belonging to one of the most enriched signaling pathways are marked in color. d Matrix showing the normalized mRNA expression levels of those genes that belong to the DNA replication signaling pathway. e qPCR validation experiments of selected genes on the sensitized + Not3 defective tumor models. Data were normalized accordingly to the expression levels on the sensitized + UAS-RNAi white eye-antennal discs. Results are plotted as mean ± standard deviation. f qPCR validation experiments of selected genes on the ey-Gal4 Not3 defective models. Data were normalized accordingly to the expression levels on the ey-Gal4 + UAS-RNAi white eye-antennal discs. Results are plotted as mean ± standard deviation

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