Fig. 6

Upregulation of cycB contributes to the tumor phenotype in Not3 and twin sensitized tumor models. a Sensitized + Not3 defective tumor model and sensitized + twin defective tumor model. Bar graphs showing rescue scoring results for tumor model or tumor plus knockdown for the indicated gene. Two independent RNAis/mutant lines are shown. Three independent crosses were screened on each experimental condition. The mean number of eyes analyzed is shown on the graph (Y-axis). Genetic suppression of os and upd3 on the sensitized + Not3 tumor animals caused pupal lethality precluding its analysis. Blue, no hyperplasia; yellow, score 1: 1 fold; light orange, score 2: 2–4 folds; dark orange, no differentiation; gray, no data available. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. b Quantification of 10xSTAT92E-GFP expression domains on the posterior part of L3 wandering larval eye-antennal discs using the color inspector 3D ImageJ plugin. GFP expression domains were measured by analysis of confocal micrographs and expressed as a percentage of the posterior eye-disc area. Bar graphs show the percentage of areas positive for GFP signal (thus, areas with JAK-STAT signaling activation) from the different genotypes; ** p < 0.01. Six eye-antennal discs were analyzed per experimental condition. Experimental crosses were performed in triplicate. Graph shows results from one representative experiment. c Dot graphs show the green signal intensity of those areas previously selected as GFP positive areas. We observed that both the area of GFP expression domains and the green intensity of those areas are increased in sensitized + Not3 (n = 6) or sensitized + twin tumor eye-antennal discs (n = 6). Experimental crosses were performed in triplicate; graph shows results from one representative experiment. d Representative confocal images of eye-antennal imaginal discs of the indicated genotypes. Green signal: 10xSTAT92E-GFP; red signal: ELAV protein; white signal: pH3 positive cells. Scale bars are 50 μM