Fig. 2

NEAT1 promoted CRC cell proliferation in vitro and in vivo. a The baseline NEAT1 RNA levels in eight CRC cell lines detected by RT-qPCR. b The efficiency of NEAT1 knockdown or overexpression was detected by RT-qPCR in the indicated cells transfected with siRNAs or plasmids (*p < 0.05). c CCK8 and d EdU assays showing that knockdown of NEAT1 suppressed cell proliferation in the HCT116 and SW1116 cell lines (*p < 0.05) and that upregulation of NEAT1 promoted cell proliferation in the HT29 cell line (*p < 0.05). e Colony formation assays for the indicated cells after transfection with siRNAs or plasmids (*p < 0.05). The nude mouse xenograft model showed that knockdown of NEAT1 decreased tumor growth (f) and the tumor weights (g) compared with those of the HCT116-shNC cells (*p < 0.05). h Representative images of tumors in nude mice. i Representative images of IHC staining for ki-67 and DDX5 (Sc represents scramble)