Fig. 4

NEAT1 binds to the DDX5 protein and enhances its stability. a RNA pull-down assay after silver staining and b western blotting to detect DDX5 protein expression in HCT116 cells. c RIP assay showing that DDX5 interacted with NEAT1 in HCT116 cells. The RT-qPCR products were analyzed by electrophoresis (below) (*p < 0.05). d The DDDX5 mRNA and e protein levels after transfection of the cells with siRNAs (*p < 0.05). f Dual-Luciferase Assays to assess DDX5 promoter activity. g Western blotting showing the DDX5 protein level after treatment of the cells with CHX (50 μg/mL) (*p < 0.05). h Western blotting showing the DDX5 protein level after treatment of the cells with or without MG132 (10 μmol/mL)