Fig. 2

2B4 and 1D12 inhibit vWF binding. a The vWF binding was inhibited by 2B4 and 1D12 and detected by flow cytometry. Washed mouse platelets were incubated with 10 μg/ml 2B4 or 10 μg/ml 1D12 for 20 min, and then 2 μg/ml recombined mouse vWF was added in the presence of 1 mg/ml ristocetin. Binding of vWF was detected with FITC-conjugated mouse vWF IgG by flow cytometry and quantitated by mean fluorescence intensity. b 2B4 inhibited ristocetin- and collagen-induced platelet aggregation. Different agonist-induced aggregation of PRP that had been pretreated with 10 μg/ml rat IgG (negative control, gray), 10 μg/ml 2B4 (blue), and 10 μg/ml 1D12 (orange) were detected for 6 min. Agonists: ristocetin (1 mg/ml), thrombin (0.05 U/ml), ADP (10 nM), and collagen (2 μg/ml). The histograms are representative of three independent experiments