Fig. 2

Expression of notch ligands in ATL cell lines. a PCR on Tax and GADPH expression from cDNA derived from ATL-derived cell lines and negative controls, Jurkat and PBMCs. GAPDH expression was used as an internal control. Real-time PCR was performed on JAG1 (b) or DLL4 (e) using cDNA from ATL-derived cell lines (ATLT, KK1, SO4, KOB, LM-Y1, ATL55T, ATL-5, ATL43T, ED-40515(−), and Tl-Om1). HTLV-I-negative Jurkat T cell line and normal PBMCs isolated from HTLV-1-negative donors were used as controls. Real-time PCR was performed in duplicate, and samples were normalized to GAPDH expression. Fold change was calculated by comparing values with Jurkat normalized JAG1 expression. Antibody staining of JAG1 (c) or DLL4 (f) was performed on the ATL cell lines, ATL55T and ATLT, and Jurkat (data included in Fig. 1). Cells were stained with the antibody against JAG1 and analyzed via a flow cytometer. FITC Mouse IgG2a isotype was used as an internal control. Red peaks indicate the control, while blue peaks indicate JAG1 or DLL4 antibodies. Bar diagrams representing the FACS results are provided. Immunohistochemistry of JAG1 on ATL55T and ATLT (d) or DLL4 on ATL25 and ATLT (g) and Jurkat as a negative control was performed. Images were taken with a × 60 objective lens