Fig. 3From: JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cellsHTLV-I, Tax, induces expression of JAG1 through NF-κB. a PCR was performed to detect Tax expression on cDNA derived from the ALL cell line, Molt4, infected with the lentivirus pSIH1-Tax. Cells infected with the empty vector pSIH1. b Antibody staining of JAG1 and DLL4 was performed on Molt4 cells infected with pSIH1-Tax and pSIH1-GFP. Red peaks indicate the control, while blue peaks indicate the JAG1 or DLL4 antibodies. Bar diagrams representing the FACS results are provided. c Real-time PCR of JAG1 and DLL4 expression on cDNA derived from Molt4 infected with pSIH1-Tax. Cells infected with the empty vector expressing pSIH1-GFP were used as a control. The expression of JAG1 and DLL4 were normalized to GAPDH expression. Results were plotted as mean ± standard deviation from at least two independent experiments. d PCR was performed to detect HBZ expression on cDNA derived from Molt4 infected with a lentivirus pSIH1-HBZ. Cells infected with the empty vector expressing pSIH1-GFP were used as a control. GAPDH expression was used as an internal control. e Antibody staining of JAG1 and DLL4 was performed on the Molt4 cells infected pSIH1-HBZ and pSIH1-GFP. Red peaks indicate the control, while blue peaks indicate the JAG1 or DLL4 antibodies. Bar diagrams representing the FACS results are provided. f Real-time PCR to detect JAG1 and DLL4 expression on cDNA extracted from Molt4 cells infected with pSIH1-HBZ. Cells infected with the empty vector expressing pSIH1-GFP were used as a control. Real-time PCR was performed in duplicate, and samples were normalized to GAPDH expression. Results were plotted as mean ± standard deviation from at least two independent experiments. g Real-time PCR analysis of IL-8, JAG1, and Hes-1 expression on cDNA from Molt4 cells infected with pSIHI-GFP and a lentivirus vector expressing IκB-α-DN mutant. Cells infected with the empty vector expressing pSIH1-GFP were used as a control. Extracts were analyzed 48 h after infection and normalized to GAPDH expression. Real-time PCR was performed in duplicate. Results were plotted as mean ± standard deviation from two independent experiments. h NF-κB luciferase was performed on 293T cells transfected with Tax and/or IκBα-DN plasmidsBack to article page