Fig. 5

Primary ATL patients overexpress JAG1, which correlates with STAT3 and NFATc1. a Real-time PCR analysis of JAG1 expression from cDNA derived from uncultured PBMCs of ATL patients and HTLV-1-negative donors. Samples were normalized to GAPDH expression, and the fold change was calculated by comparing values to healthy donor(s) normalized gene expression. b Real-time PCR analysis of JAG1, IL-8, RelA, STAT3, and NFATc1 expression from cDNA derived from PBMCs of patients with ATL and HTLV-1-negative donors. Samples were normalized to GAPDH expression, and the fold change was calculated by comparing values to healthy donor normalized gene expression. Correlation analysis was performed on JAG1 versus RelA, IL-8 versus RelA, JAG1 versus STAT3, and JAG1 versus NFATC1 in ATL patients. The Pearson’s correlation coefficient (r), coefficient of determination (R²), and p value of the Pearson’s correlation coefficient are reported. c, d ED-40515(−) cells were treated 24 h with 50 μM S3I-201 (c) or 48 h with 100 μM NFAT inhibitor (inNFAT) (d). RT-PCR was performed on JAG1 expression, normalized to GAPDH control. For inNFAT, expression of NFATC1 was also noted. Experiments were performed at least twice, and fold change was calculated from the average repression compared to DMSO control samples